🧫
ARF6 and exocyst complex role in VE-cadherin trafficking
active
experiment
Created: 2026-04-06T12:34:46
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-100f96da-93d1-4b84-9f92-2cc9a56230a0
🧫 Experiment Protocol
ExploratoryARF6lymphatic endothelial cellsproposed
Following the proximity proteomics screen, the researchers conducted functional validation experiments to characterize the roles of specific identified interactors, particularly ADP ribosylation factor 6 (ARF6) and the exocyst complex, in VE-cadherin trafficking and recycling. These experiments likely involved cell biological approaches such as fluorescence microscopy, protein localization studies, and functional assays to demonstrate how these proteins contribute to VE-cadherin dynamics at adherens junctions. The study aimed to understand the molecular mechanisms by which these interactors regulate VE-cadherin trafficking between different cellular compartments and its recycling back to junctions.
PRIMARY OUTCOME
demonstration of ARF6 and exocyst complex roles in VE-cadherin trafficking
EXPECTED OUTCOMES
evidence for ARF6 and exocyst complex involvement in VE-cadherin trafficking and recycling
SUCCESS CRITERIA
demonstration of functional roles for identified interactors in VE-cadherin dynamics
PROTOCOL
cell biological assays including microscopy and functional validation experiments
Source: PMID 39232006 ↗
🧫 Experiment Extras
PATHWAY
protein trafficking and recycling pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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