🧫
Microglial depletion study using clodronate liposomes
active
experiment
Created: 2026-04-10T22:34:12
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-169f2235-b61b-484a-889d-e3d5296755a1
🧫 Experiment Protocol
Exploratoryretinitis pigmentosaPde6brd10 mouse organotypic retinal explants with microglial depletionproposed
This experiment investigated the role of microglia in IGF-I-mediated neuroprotection by depleting microglial cells using clodronate-containing liposomes in organotypic retinal explants from rd10 mice. The study compared photoreceptor cell death in explants treated with IGF-I in the presence and absence of microglia. Results showed that microglial depletion diminished the neuroprotective effect of IGF-I, indicating that microglia are required for IGF-I's protective function. Interestingly, microglial depletion alone also moderately reduced photoreceptor cell death in untreated rd10 retinas, suggesting a complex dual role for microglia in retinal degeneration.
PRIMARY OUTCOME
microglial requirement for IGF-I neuroprotection
EXPECTED OUTCOMES
Microglial depletion would affect IGF-I's neuroprotective capacity
SUCCESS CRITERIA
Changes in IGF-I neuroprotective effect upon microglial depletion
PROTOCOL
Treatment with clodronate-containing liposomes for microglial depletion, IGF-I treatment, TUNEL staining, comparison of cell death with and without microglia
Source: PMID 22039242 ↗
🧫 Experiment Extras
PATHWAY
microglial activation and IGF-I signaling
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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