🧫
Irisin treatment in mouse femur fracture healing model
active
experiment
Created: 2026-04-10T14:42:15
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-4a5ec8eb-56b5-4502-9082-f61e4c8ca34f
🧫 Experiment Protocol
Validationbone fractureC57BL/6 miceproposed
In vivo study examining the effects of irisin on bone fracture healing using a mouse femur fracture model. Closed mid-diaphyseal femur fractures were created in 8-week-old C57BL/6 mice, followed by intraperitoneal irisin administration every other day post-surgery. Fracture healing was assessed through multiple approaches including X-ray imaging, micro-computed tomography for bone morphometry of fracture callus, and three-point bending mechanical testing of femurs. The study evaluated callus formation, mineralization, vessel surface and volume fraction, and expression of key genes including BMP2, CD31, and VEGF in the callus tissue. Results demonstrated enhanced callus formation, increased mineralization, improved mechanical properties, and enhanced vascularization in irisin-treated mice compared to controls.
PRIMARY OUTCOME
fracture healing assessed by X-ray, micro-CT, and mechanical testing
EXPECTED OUTCOMES
improved fracture healing with irisin treatment
SUCCESS CRITERIA
increased callus formation, mineralization, and mechanical strength
PROTOCOL
Closed mid-diaphyseal femur fractures created, irisin administered intraperitoneally every other day, assessment by X-rays, micro-CT bone morphometry, three-point bending testing
Source: PMID 36196152 ↗
🧫 Experiment Extras
PATHWAY
bone formation/osteogenesis and angiogenesis pathways
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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