Direct targeting of Capza1 by silibinin

🧫 Experiment Protocol Exploratorybreast cancerCAPZA1, YAP1MCF-7 and MDA-MB-231 human breast cancer cell linesproposed

This experiment focused on identifying the direct molecular target of silibinin that mediates F-actin disassembly. The researchers used biochemical approaches to investigate the interaction between silibinin and F-actin regulatory proteins. They discovered that silibinin directly targets Capza1 (capping actin protein of muscle Z-line subunit alpha 1), which is responsible for F-actin disassembly. The study likely employed techniques such as CETSA (cellular thermal shift assay) and DARTS (drug affinity responsive target stability) to demonstrate direct drug-protein interactions. The researchers showed that Capza1-mediated F-actin disassembly is the decisive mechanism for silibinin-induced cell cycle arrest, even when YAP activity is modulated. This experiment revealed a positive feedback loop between YAP and F-actin, where promoting F-actin assembly through si-Capza1 transfection can restore YAP activity.

Source: PMID 41349910 ↗

🧫 Experiment Extras

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