🧫
In vitro PANoptosis protection by LPT1 in hepatocytes
active
experiment
Created: 2026-04-10T22:20:51
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-94dcfc3e-bfb5-4048-94ec-f9207e6ed791
🧫 Experiment Protocol
Exploratorymetabolic dysfunction-associated fatty liver disease (MAFLD)cultured hepatocytesproposed
This in vitro experiment tested whether LPT1 could protect cultured hepatocytes against cell death induced by pro-PANoptosis molecules under lipid stress conditions. Hepatocytes were treated with LPT1 (100 nM) and then challenged with PANoptosis-inducing agents including TNF-α, LPS, and nigericin in the presence of lipid stress to create an in vitro MAFLD model. The experiment aimed to validate the protective effects of LPT1 observed in the mouse model and specifically test its ability to prevent PANoptosis-mediated cell death in hepatocytes. This complemented the in vivo findings by providing mechanistic insights into how LPT1 protects against the coordinated cell death pathway involving apoptosis, pyroptosis, and necroptosis.
PRIMARY OUTCOME
cell viability under PANoptosis-inducing conditions
EXPECTED OUTCOMES
LPT1 would prevent cell death induced by PANoptosis stimuli
SUCCESS CRITERIA
improved cell survival compared to untreated controls
PROTOCOL
hepatocytes treated with LPT1 (100 nM) then challenged with TNF-α, LPS, and nigericin under lipid stress
Source: PMID 36323829 ↗
🧫 Experiment Extras
PATHWAY
PANoptosis, apoptosis, pyroptosis, necroptosis
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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