🧫
ZG efficacy assessment in PCPA-induced insomnia-anxiety mice
active
experiment
Created: 2026-04-10T22:36:30
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-95b6c13e-2092-46ce-902f-7d45cd20e205
🧫 Experiment Protocol
Validationinsomnia-anxiety comorbidityPCPA-treated miceproposed
Mice were administered 4-chloro-DL-phenylalanine (PCPA) to induce insomnia and anxiety-like behaviors, then treated with Zhi-Gan Formula (ZG) or diazepam for comparison. Behavioral assessments were conducted using pentobarbital-induced sleep test to measure sleep latency and duration, open-field test (OFT) and elevated plus-maze test (EPM) to evaluate anxiety-like behaviors. This experiment aimed to demonstrate ZG's therapeutic efficacy for comorbid insomnia and anxiety conditions compared to standard pharmacological treatment.
PRIMARY OUTCOME
sleep latency, sleep duration, and anxiety-like behaviors
EXPECTED OUTCOMES
ZG would improve both sleep parameters and anxiety-like behaviors better than diazepam
SUCCESS CRITERIA
Significant reduction in sleep latency, increased sleep duration, and decreased anxiety-like behaviors
PROTOCOL
PCPA injection followed by 3-day ZG or diazepam treatment, behavioral testing with pentobarbital-induced sleep test, OFT, and EPM
Source: PMID 41525914 ↗
🧫 Experiment Extras
PATHWAY
neuropeptide-receptor interactions and synaptic transmission pathways
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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