🧫
BDEV injection and recipient microglial transcriptome analysis
active
experiment
Created: 2026-04-12T18:03:52
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-a7eb292f-c0d7-497d-a55b-f16ea8ab34b2
🧫 Experiment Protocol
ValidationAlzheimer's disease/tauopathyP2rx7Recipient mice injected with BDEVs from PS19 miceproposed
This experiment involved injecting brain-derived extracellular vesicles (BDEVs) isolated from PS19 mice (with or without P2rx7 deficiency) into recipient mice, followed by single-cell RNA sequencing analysis of microglial responses. The study aimed to determine how P2rx7-containing EVs affect microglial gene expression and inflammatory responses in recipient animals. Researchers likely performed stereotactic injections of purified BDEVs into specific brain regions, followed by tissue harvest and single-cell RNA sequencing focusing on microglial populations. The transcriptomic analysis revealed enrichment of DNA-sensing and type II interferon signaling pathways in response to P2rx7-containing EVs.
PRIMARY OUTCOME
Microglial transcriptomic changes and inflammatory pathway activation
EXPECTED OUTCOMES
BDEVs from P2rx7-expressing mice would induce stronger inflammatory responses in recipient microglia
SUCCESS CRITERIA
Enrichment of DNA-sensing and interferon signaling pathways in response to P2rx7-containing BDEVs
PROTOCOL
Stereotactic injection of BDEVs, single-cell RNA sequencing of recipient brain tissue, pathway enrichment analysis
LINKED HYPOTHESES
Source: PMID 40678243 ↗
🧫 Experiment Extras
PATHWAY
DNA-sensing pathways and type II interferon signaling in microglia
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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