🧫
CarH3 Degradation and Anti-CarP Antibody Protection Assay
active
experiment
Created: 2026-04-10T23:14:19
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-c4a71222-d85b-4dfd-abc6-037f251d29c2
🧫 Experiment Protocol
Exploratoryrheumatoid arthritishistone H3in vitro protein degradation assayproposed
This experiment evaluated the degradation kinetics of carbamylated histone H3 (carH3) by neutrophil elastase and tested whether anti-carbamylated protein (anti-CarP) IgG antibodies from rheumatoid arthritis patients could protect carH3 from proteolytic degradation. The study examined how antibody binding affects the stability of carbamylated proteins, which has important implications for autoantigen persistence in inflamed joints. The experiment involved incubating carH3 with neutrophil elastase in the presence or absence of patient-derived anti-CarP antibodies and measuring the remaining intact protein over time.
PRIMARY OUTCOME
protection of carH3 from neutrophil elastase degradation by anti-CarP antibodies
EXPECTED OUTCOMES
anti-CarP antibodies stabilize carH3 against proteolytic degradation
SUCCESS CRITERIA
reduced carH3 degradation in presence of anti-CarP antibodies
PROTOCOL
protein degradation kinetics assay, neutrophil elastase treatment, anti-CarP IgG incubation
Source: PMID 41930627 ↗
🧫 Experiment Extras
PATHWAY
protein degradation, immune complex formation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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