🧫
Butyrate rescue experiment in germfree colonocytes
active
experiment
Created: 2026-04-10T23:13:52
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-cc2ca8ba-5268-4135-970a-260a95c11c56
🧫 Experiment Protocol
Exploratorygermfree mouse colonocytesproposed
This experiment involved adding butyrate to colonocytes isolated from germfree mice to test whether this bacterial metabolite could rescue the observed metabolic deficits. The study measured mitochondrial respiration capacity and autophagy markers before and after butyrate treatment. The experiment was designed to determine whether butyrate functions primarily as an energy source for colonocytes or as an HDAC inhibitor. The results showed that butyrate supplementation restored mitochondrial respiration and prevented autophagy in germfree colonocytes, demonstrating that bacterial butyrate is essential for colonocyte energy metabolism and that its primary mechanism is as an energy substrate rather than through epigenetic regulation.
PRIMARY OUTCOME
Mitochondrial respiration capacity and autophagy prevention
EXPECTED OUTCOMES
Butyrate would rescue mitochondrial respiration deficits and prevent autophagy in germfree colonocytes
SUCCESS CRITERIA
Restoration of normal mitochondrial respiration and prevention of autophagy following butyrate treatment
PROTOCOL
Treatment of germfree colonocytes with butyrate followed by measurement of mitochondrial respiration and autophagy markers
Source: PMID 21531334 ↗
🧫 Experiment Extras
PATHWAY
mitochondrial respiration, autophagy
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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