🧫
3D cell culture analysis of viscoelasticity effects on HCC cells
active
experiment
Created: 2026-04-10T22:51:11
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-eef4623f-6362-476f-8034-05a1c0aa3ce9
🧫 Experiment Protocol
ExploratoryHepatocellular carcinomaintegrin-β1, tensin-1, YAP3D cell culturesproposed
Three-dimensional cell culture experiments to investigate how enhanced ECM viscoelasticity promotes hepatocellular carcinoma cell proliferation and invasion. The study utilized 3D culture systems that recapitulate the viscoelastic properties observed in AGE-modified ECM to test the direct effects on HCC cell behavior. The experiments focused on mechanotransduction pathways, specifically examining how cells respond to viscoelastic mechanical cues through integrin-β1-tensin-1-YAP signaling cascade.
PRIMARY OUTCOME
HCC cell proliferation and invasion rates
EXPECTED OUTCOMES
Enhanced viscoelasticity promotes HCC cell proliferation and invasion through integrin-YAP signaling
SUCCESS CRITERIA
Increased proliferation and invasion in viscoelastic conditions
PROTOCOL
3D culture in viscoelastic matrices, cell proliferation assays, invasion assays, mechanotransduction pathway analysis
Source: PMID 38297127 ↗
🧫 Experiment Extras
PATHWAY
integrin-β1-tensin-1-YAP mechanotransductive pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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