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Fig. 1 — Lipidome and proteome of astrocyte and microglia ApoE lipoprotein reveal differe
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Created: 2026-04-21T18:29:40
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Fig. 1Figure 1
A: Schematic showing workflow for purification of intact ApoE lipoprotein particles, followed by proteomic and lipidomic analyses. Primary glia were first isolated from the cortex of P2–P3 pups. Mixed glial cultures were then shaken to isolate primary microglia cultures and primary astrocyte cultures. Cultures were switched to serum-free media prior to collection of media. Astrocyte or microglia-conditioned media were flowed over immunoaffinity columns to isolate ApoE lipoproteins. Intact particles were then assessed using MS-based lipidomics and proteomics. B: Nonreducing SDS-PAGE gel shows that ApoE2 and ApoE3 form disulfide-linked dimers in lipidated ApoE. ApoE4, which does not contain a Cys residue, does not. An SDS-resistant dimer is seen; however, this is resistant to the addition of DTT (Fig. 1C), suggesting that it is not a disulfide-linked dimer. The absence of signal in ApoE −/− samples shows that no ApoE is present in samples collected from ApoE −/− astrocyte or microglia-
▸Metadata
| pmid | paper-41692246 |
| caption | A: Schematic showing workflow for purification of intact ApoE lipoprotein particles, followed by proteomic and lipidomic analyses. Primary glia were first isolated from the cortex of P2–P3 pups. Mixed |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC12996666/bin/gr1.jpg |
| paper_title | Lipidome and proteome of astrocyte and microglia ApoE lipoprotein reveal differences based on cell type and ApoE isoform. |
| figure_label | Fig. 1 |
| figure_number | 1 |
| _schema_version | 1 |
| source_strategy | pmc_api |
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