🧫
dilncRNA-mediated molecular crowding and phase separation
active
experiment
Created: 2026-04-06T12:31:13
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-065d236a-372a-427f-90ca-630df955d58c
🧫 Experiment Protocol
Exploratorymammalian cellsproposed
This experiment investigated the mechanism by which damage-induced long non-coding RNAs (dilncRNAs) promote the concentration of DDR proteins into foci. The researchers demonstrated that dilncRNAs drive molecular crowding of DDR proteins such as 53BP1 into condensates that exhibit properties of liquid-liquid phase separation. This study provided mechanistic insight into how RNA synthesis at DSB sites contributes to the physical organization of DDR factors into discrete, membrane-less compartments with specific biophysical properties.
PRIMARY OUTCOME
Phase separation properties of DDR protein condensates
EXPECTED OUTCOMES
dilncRNAs promote formation of DDR protein condensates with phase separation characteristics
SUCCESS CRITERIA
Demonstration of liquid-liquid phase separation properties in DDR foci and dependence on dilncRNA
PROTOCOL
Analysis of dilncRNA effects on DDR protein condensation and characterization of liquid-liquid phase separation properties
Source: PMID 31570834 ↗
🧫 Experiment Extras
PATHWAY
DNA damage response pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.