🧫
Factor depletion effects on DDR focus formation in vivo
active
experiment
Created: 2026-04-06T12:31:13
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-0afb764b-a8a7-4666-b2ce-d5c1909cc260
🧫 Experiment Protocol
Exploratorymammalian cells in vivoproposed
This experiment examined the consequences of depleting or inactivating key transcriptional factors (RNA polymerase II, MED1, CDK9) on DNA damage response (DDR) focus formation in living cells. The researchers used loss-of-function approaches to demonstrate that these transcriptional components are necessary for proper DDR focus formation. The study showed that when these factors were absent or inactive, there was a significant reduction in the formation of DDR foci containing proteins like 53BP1.
PRIMARY OUTCOME
DDR focus formation efficiency
EXPECTED OUTCOMES
Reduced DDR focus formation upon factor depletion
SUCCESS CRITERIA
Statistically significant reduction in DDR foci when transcriptional factors are depleted
PROTOCOL
Depletion or inactivation of RNA polymerase II, MED1, and CDK9 followed by assessment of DDR foci formation
Source: PMID 31570834 ↗
🧫 Experiment Extras
PATHWAY
DNA damage response pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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