scRNA-seq analysis of human atherosclerotic plaques

Single-cell RNA sequencing analysis was performed on human atherosclerotic plaque samples to identify cell clusters and complement C1Q-related differentially expressed genes. The analysis identified 24 cell clusters and 12 cell types from the scRNA-seq dataset (GSE159677). Seven C1Q-associated differentially expressed genes were identified in both the scRNA-seq and bulk RNA-seq datasets. Machine learning algorithms including GBM, LASSO, and XGBoost were used to select C1QA and C1QC as hub genes from the seven DEGs. The study also identified SPI1 as a potential transcription factor regulating these hub genes in human atherosclerotic plaques.