🧫
NET Generation and Carbamylation Analysis
active
experiment
Created: 2026-04-10T23:14:19
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2587c1f7-ba19-4687-9a17-31e86597c639
🧫 Experiment Protocol
Exploratoryrheumatoid arthritishuman neutrophilsproposed
Neutrophil extracellular traps (NETs) were generated using calcium ionophore treatment and analyzed for carbamylation modifications. The study examined histone carbamylation, particularly of histone H3, during NETosis using multiple detection methods including immunofluorescence microscopy, flow cytometry, and mass spectrometry. The experiment aimed to characterize the extent and specificity of protein carbamylation that occurs during NET formation, with particular focus on identifying carbamylated histone H3 (carH3) as a major carbamylated autoantigen produced during this process.
PRIMARY OUTCOME
histone carbamylation levels during NETosis
EXPECTED OUTCOMES
carbamylation of histones, particularly H3, during NET formation
SUCCESS CRITERIA
detection of carbamylated proteins in NETs
PROTOCOL
calcium ionophore stimulation, immunofluorescence, flow cytometry, mass spectrometry
Source: PMID 41930627 ↗
🧫 Experiment Extras
PATHWAY
NETosis/neutrophil activation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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