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Analysis of MLIV patient fibroblasts for contact dynamics and calcium uptake
active
experiment
Created: 2026-04-10T14:43:41
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-38e8744d-9cf1-438e-9c66-cb56da8b6a1c
🧫 Experiment Protocol
Clinicalmucolipidosis type IVMCOLN1human patient fibroblastsproposed
Patient-derived fibroblasts from individuals with mucolipidosis type IV (MLIV) were analyzed to examine mitochondria-lysosome contact dynamics and contact-dependent mitochondrial calcium uptake. Since MLIV results from loss of TRPML1 function, these patient cells served as a disease model to validate the functional importance of TRPML1 in mitochondria-lysosome contact-mediated calcium transfer. The study compared contact site formation, stability, and calcium transfer efficiency between MLIV patient fibroblasts and control cells. This experiment aimed to demonstrate the pathophysiological relevance of the mitochondria-lysosome contact mechanism discovered in the primary cell biology experiments and to show how TRPML1 dysfunction in human disease affects these critical interorganelle interactions.
PRIMARY OUTCOME
mitochondria-lysosome contact dynamics and calcium uptake defects
EXPECTED OUTCOMES
MLIV patient cells should show altered contact dynamics and defective calcium transfer
SUCCESS CRITERIA
measurable differences in contact formation and calcium uptake between patient and control cells
PROTOCOL
comparison of patient vs control fibroblasts using live-cell microscopy and calcium imaging
LINKED HYPOTHESES
Source: PMID 32703809 ↗
🧫 Experiment Extras
PATHWAY
mitochondrial calcium signaling
MARKET PRICE
$0.50
STATUS
proposed
Prediction Markets (1 direct, 0 via hypothesis — 1 total)
Analysis of MLIV patient fibroblasts for contact dynamics and calcium uptake — will this eYES 80% · Liq $100 · active▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
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Certainty
0%
Debates
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Incoming
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Outgoing
0
0 supporting
0 contradicting
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