🧫
Multi-omics analysis of myricanol effects on gene expression and microRNA regulation
active
experiment
Created: 2026-04-10T22:46:31
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-3b7faafc-4bd9-407f-ae87-8cfbd6237e51
🧫 Experiment Protocol
ExploratoryhyperlipidemiaACSL1, PPARγ, CYP7A1, SCD1, ACLY, SREBF1, CPT1A, ACC1, APOE4C57BL/6J mouse liver and epididymal fat tissueproposed
This comprehensive gene expression study utilized RNA sequencing and metabolomics to investigate the molecular mechanisms underlying myricanol's lipid-lowering effects. The analysis revealed that myricanol treatment significantly altered the expression of key metabolic genes in liver tissue, including upregulation of ACSL1, PPARγ, CYP7A1, SCD1, ACLY, and SREBF1. In epididymal fat tissue, myricanol increased expression of PPARγ, CPT1A, ACC1, ACSL1, APOE4, and SREBF1. The study also identified regulation of specific microRNAs including miR-203b-3p, miR-205-5p, and miR-184-3p. Additionally, pathway analysis revealed modulation of the interferon (IFN) pathway and IFN-stimulated genes. The multi-omics approach provided systems-level insight into how myricanol orchestrates metabolic reprogramming to achieve its therapeutic effects, demonstrating coordinate regulation of lipid synthesis, oxidation, and transport pathways.
PRIMARY OUTCOME
gene expression changes in metabolic pathways and microRNA regulation
EXPECTED OUTCOMES
Upregulation of beneficial metabolic genes, modulation of lipid metabolism pathways, altered microRNA expression profiles
SUCCESS CRITERIA
Significant differential expression of target genes, pathway enrichment in lipid metabolism, coordinated regulation of metabolic networks
PROTOCOL
RNA sequencing for transcriptome analysis, metabolomics for metabolite profiling, pathway enrichment analysis for functional annotation
Source: PMID 41520958 ↗
🧫 Experiment Extras
PATHWAY
lipid metabolism, interferon signaling pathway, microRNA regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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