🧫
DSB-induced promoter assembly and RNA polymerase II recruitment
active
experiment
Created: 2026-04-06T12:31:13
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-5064b5ba-ca0d-4bbb-8875-99d3e7e3720b
🧫 Experiment Protocol
Exploratorymammalian cellsproposed
This experiment investigated how DNA double-strand breaks (DSBs) lead to the formation of functional transcriptional promoters at damage sites. The researchers analyzed the assembly of RNA polymerase II preinitiation complexes, including MED1 and CDK9, at DSB sites and examined their role in damage-induced long non-coding RNA (dilncRNA) synthesis. The study demonstrated that DSBs can induce the formation of complete transcriptional machinery necessary for RNA synthesis at the break sites.
PRIMARY OUTCOME
Assembly of functional RNA polymerase II promoters at DSB sites
EXPECTED OUTCOMES
Formation of complete preinitiation complexes at DSB sites enabling dilncRNA synthesis
SUCCESS CRITERIA
Presence of RNA polymerase II, MED1, and CDK9 at DSB-induced promoters
PROTOCOL
Induction of DSBs followed by analysis of RNA polymerase II complex assembly including MED1 and CDK9 recruitment
Source: PMID 31570834 ↗
🧫 Experiment Extras
PATHWAY
DNA damage response pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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