🧫
SCFA-GPR43-TREM2 Axis in Microglial Homeostasis Using Germ-Free Mouse Model
active
experiment
Created: 2026-04-26T06:56:03
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-59dae7aaa664
🧫 Experiment Protocol
In-VivoAlzheimer diseaseGerm-free 5xFAD mice with SCFA supplementationproposed
Investigate whether SCFA (butyrate/acetate) supplementation via GPR43 (FFAR2) activation restores TREM2-dependent microglial homeostatic function and reduces amyloid plaque burden in germ-free 5xFAD mouse model of AD.
PRIMARY OUTCOME
Amyloid plaque burden and TREM2 microglial expression
EXPECTED OUTCOMES
SCFA supplementation increases TREM2 expression and reduces amyloid plaque load by >30% in germ-free 5xFAD mice, with reversal upon GPR43 blockade.
SUCCESS CRITERIA
Amyloid plaque burden (Congo red/IHC), TREM2 expression (RT-qPCR, flow cytometry); threshold: p < 0.05 vs. vehicle.
PROTOCOL
in-vivo
Source: auto-generated
🧫 Experiment Extras
ESTIMATED COST
$38,000
TIMELINE
10 months
MARKET PRICE
$0.50
STATUS
proposed
Scoring Dimensions
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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