🧫
Tau depletion effects on neuronal development in primary cultures
active
experiment
Created: 2026-04-06T12:32:24
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-65257309-482b-4700-8d80-284109e46653
🧫 Experiment Protocol
ExploratoryMaptprimary rodent neuronal culturesproposed
This experiment examined the effects of tau protein depletion on various aspects of neuronal development using primary rodent neuronal cultures. The study investigated how loss of tau affects the rate of neuronal development, growth cone turning efficiency, and the formation of neuronal processes and axonal branches. Since tau association with microtubules makes them labile (unstable), the researchers hypothesized that tau depletion would alter neuronal morphology and development through changes in microtubule dynamics. The experiment involved depleting tau from cultured neurons and measuring various developmental parameters to understand tau's role in neuronal morphogenesis.
PRIMARY OUTCOME
rate of neuronal development, growth cone turning efficiency, neuronal process number, axonal branch number
EXPECTED OUTCOMES
altered neuronal development parameters due to changes in microtubule stability
SUCCESS CRITERIA
measurable changes in developmental metrics compared to control
PROTOCOL
tau depletion in primary neuronal cultures with measurement of developmental parameters
LINKED HYPOTHESES
h-23b94ed8· Locus Coeruleus-Hippocampal Circuit Protectionh-var-f687d4593b· Cholinergic Basal Forebrain-Hippocampal Circuit Protectionh-var-bc4357c8c5· Dopaminergic Ventral Tegmental-Hippocampal Circuit Protectionh-var-8412ce00a4· Dual-Circuit Tau Vulnerability Cascadeh-var-95b0f9a6bc· Glymphatic-Mediated Tau Clearance Dysfunction
Source: PMID 39257379 ↗
🧫 Experiment Extras
PATHWAY
microtubule dynamics
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.