🧫
FK866 mechanism validation in Rag1-/- mice
active
experiment
Created: 2026-04-10T14:44:04
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-7083c4d7-bc53-40b1-9d97-6340b092c977
🧫 Experiment Protocol
ValidationInflammatory bowel diseaseNAMPTRag1-/- mice with DSS-induced colitisproposed
Mechanistic validation experiment using Rag1-/- mice (lacking mature T and B cells) to determine whether FK866's therapeutic effects are primarily mediated through innate immune cells rather than adaptive immunity. This experiment was crucial for establishing that FK866's anti-inflammatory effects are independent of T and B cell responses and are primarily mediated through altered monocyte/macrophage biology. The effectiveness of FK866 in Rag1-/- mice confirmed that the therapeutic mechanism involves direct modulation of innate immune cell function, particularly macrophage polarization, rather than effects on adaptive immune responses.
PRIMARY OUTCOME
Anti-inflammatory efficacy in absence of adaptive immunity
EXPECTED OUTCOMES
Maintained anti-inflammatory efficacy demonstrating innate immune mechanism
SUCCESS CRITERIA
Significant anti-inflammatory effects in the absence of T and B cells
PROTOCOL
DSS-induced colitis in Rag1-/- mice treated with FK866, assessment of inflammatory parameters and innate immune cell responses
LINKED HYPOTHESES
Source: PMID 28877980 ↗
🧫 Experiment Extras
PATHWAY
NAD metabolism, innate immunity
MARKET PRICE
$0.50
STATUS
proposed
Prediction Markets (1 direct, 0 via hypothesis — 1 total)
TREM2 R47H Variant-Driven Metabolic Dysfunction as the Primary Trigger for Failed DAM TranYES 83% · Liq $100 · active▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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