Exploratory experiment designed to discover new patterns targeting RUBCN in PTEC-hepatocyte transwell co-culture. Primary outcome: triglyceride accumulation in hepatocytes
Performed transwell co-culture experiments with RUBCN knockout PTECs and hepatocytes (BNL-CL2 cells) to investigate fatty acid efflux. Found that KO PTECs promoted massive triglyceride accumulation in co-cultured hepatocytes, suggesting accelerated fatty acid efflux from the PTECs. This mechanism was proposed to contribute to the metabolic syndrome observed in KO mice.
Co-cultured RUBCN KO PTECs with BNL-CL2 hepatocytes in transwell system, measured triglyceride accumulation
Increased fatty acid efflux from KO PTECs leading to hepatocyte triglyceride accumulation
Quantification of triglyceride levels in hepatocytes
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