🧫
H₂O₂-induced oxidative stress in isolated chondrocytes
active
experiment
Created: 2026-04-10T22:49:51
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-8288f904-6ab6-4bbe-b9db-9855b5abbd8d
🧫 Experiment Protocol
ExploratoryTemporomandibular joint osteoarthritisCx43Isolated chondrocytesproposed
An in vitro cellular model study using isolated chondrocytes treated with hydrogen peroxide (H₂O₂) to induce oxidative stress and investigate its effects on extracellular matrix degradation, Cx43 expression, and Wnt/β-catenin signaling. This model was designed to simulate the oxidative stress conditions that occur during TMJOA progression and to study the molecular mechanisms involving Cx43 and β-catenin interactions at the cellular level.
PRIMARY OUTCOME
Oxidative stress-induced matrix degradation and increased Cx43/β-catenin expression
EXPECTED OUTCOMES
H₂O₂ treatment would trigger oxidative stress, matrix degradation, and increased expression of both Cx43 and β-catenin
SUCCESS CRITERIA
Demonstration of oxidative stress markers, matrix degradation, and upregulated Cx43 and β-catenin expression
PROTOCOL
Treatment of isolated chondrocytes with H₂O₂ to induce oxidative stress, followed by assessment of extracellular matrix degradation, Cx43 expression, and β-catenin membrane localization
Source: PMID 41757775 ↗
🧫 Experiment Extras
PATHWAY
Wnt/β-catenin signaling
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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