🧫
DSB-induced promoter assembly and RNA polymerase II recruitment
active
experiment
Created: 2026-04-06T12:30:33
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-86f850da-025c-4e40-94a1-eb39ca716a9c
🧫 Experiment Protocol
Exploratorycultured cellsproposed
This experiment investigated the formation of functional promoters at DNA double-strand breaks (DSBs) and the recruitment of RNA polymerase II transcriptional machinery. The study examined how DSBs lead to the assembly of complete preinitiation complexes including RNA polymerase II, MED1, and CDK9. The researchers likely used DNA damage induction methods to create DSBs and then analyzed the recruitment of transcriptional factors to these sites using immunofluorescence microscopy, chromatin immunoprecipitation, or similar techniques. The experiment aimed to demonstrate that DSBs can function as sites for transcriptional initiation, leading to the synthesis of damage-induced long non-coding RNAs (dilncRNAs).
PRIMARY OUTCOME
Assembly of RNA polymerase II preinitiation complex at DSBs
EXPECTED OUTCOMES
Formation of functional promoters with complete RNA polymerase II machinery at DSB sites
SUCCESS CRITERIA
Detection of RNA polymerase II, MED1, and CDK9 at DSB sites
PROTOCOL
DSB induction followed by analysis of transcriptional factor recruitment
Source: PMID 31570834 ↗
🧫 Experiment Extras
PATHWAY
DNA damage response pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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