🧫
High fat diet aging study in CPT1A knockout mice
active
experiment
Created: 2026-04-12T17:54:15
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-928d57cc-35da-45c3-a95d-e02788777e31
🧫 Experiment Protocol
Validationkidney aging and metabolic dysfunctionCPT1Aconditional CPT1A knockout miceproposed
This study examined the effects of aging combined with high fat diet (HFD) on kidney proximal tubules, specifically investigating the role of carnitine palmitoyltransferase 1A (CPT1A) in fatty acid oxidation. The experiment compared young and aged mice fed HFD, analyzing transcriptional changes in metabolism-related pathways in proximal tubules versus distal convoluted tubules (DCT). The study used conditional CPT1A knockout mice to examine the specific role of this enzyme in proximal tubule metabolism and kidney injury. Researchers measured fibrosis, inflammation, and proximal tubule cell injury, along with gene expression changes in metabolic pathways. The proximal tubules showed suppressed expression of genes related to metabolism and fatty acid oxidation with aging and HFD, while DCT showed more inflammation-related changes.
PRIMARY OUTCOME
proximal tubule injury and metabolic gene expression
EXPECTED OUTCOMES
HFD-aged mice would show increased fibrosis, inflammation, and PT injury with altered metabolism gene expression
SUCCESS CRITERIA
significant transcriptional changes in metabolism-related pathways in PT segments
PROTOCOL
HFD feeding in young vs aged mice with conditional CPT1A knockout, transcriptional analysis of kidney segments
LINKED HYPOTHESES
Source: PMID 40138521 ↗
🧫 Experiment Extras
PATHWAY
fatty acid oxidation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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