🧫
Single-cell RNA sequencing of EMT states in SCC
active
experiment
Created: 2026-04-10T14:41:33
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-a22c76d3-d586-4f64-be64-fa55688f609d
🧫 Experiment Protocol
ExploratorySkin squamous cell carcinomaMultiple EMT-related genesPrimary mouse modelproposed
This experiment used single-cell RNA sequencing to characterize different EMT states in control and NP137-treated squamous cell carcinomas. The analysis revealed the presence of distinct EMT states including epithelial, early hybrid EMT, late hybrid EMT, and full EMT states in control SCC samples. The study demonstrated that NP137 administration prevented cancer cell progression toward late EMT states and helped maintain tumor epithelial states. This comprehensive transcriptomic analysis provided molecular insights into how netrin-1 inhibition affects EMT progression at the single-cell level.
PRIMARY OUTCOME
EMT state classification and gene expression profiles
EXPECTED OUTCOMES
Prevention of late EMT state progression and maintenance of epithelial states with NP137 treatment
SUCCESS CRITERIA
Clear identification of EMT states and demonstration of NP137 effects on EMT progression
PROTOCOL
Single-cell RNA sequencing of tumor samples from control and NP137-treated mice, followed by computational analysis of EMT states
LINKED HYPOTHESES
Source: PMID 37532929 ↗
🧫 Experiment Extras
PATHWAY
EMT signaling pathways
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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