🧫
Antidepressant effects of aminophylline in chronic restraint stress mice
active
experiment
Created: 2026-04-10T22:36:02
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-a35264b6-1000-4448-a809-06a18162ac26
🧫 Experiment Protocol
Validationdepressionmice with chronic restraint stressproposed
This study evaluated the antidepressant effects of aminophylline in mice subjected to chronic restraint stress (CRS) for 15 days. The experiment involved daily 4-hour stress exposure followed by behavioral assessments to measure depressive-like behaviors. Three different doses of aminophylline (5, 10, and 20 mg/kg) were administered daily via intraperitoneal injection, with fluoxetine (10 mg/kg) serving as a positive control. Behavioral testing was conducted using three established paradigms: the tail suspension test (TST), forced swimming test (FST), and sucrose splash test (SST) to assess immobility time and grooming behaviors as indicators of depression-like phenotypes. The study demonstrated that aminophylline treatment attenuated CRS-induced behavioral deficits in a dose-dependent manner, reducing immobility time in both TST and FST while increasing grooming time in the SST.
PRIMARY OUTCOME
behavioral measures of depression-like behavior
EXPECTED OUTCOMES
reduction in immobility time and increase in grooming activity indicating antidepressant effects
SUCCESS CRITERIA
significant reduction in immobility time in TST and FST, increased grooming time in SST compared to stressed controls
PROTOCOL
15-day chronic restraint stress protocol with 4-hour daily stress exposure, daily intraperitoneal injections of aminophylline or fluoxetine, behavioral testing on days 0, 5, 10, and 15 using TST, FST, and SST
Source: PMID 41945313 ↗
🧫 Experiment Extras
PATHWAY
PPAR signaling, calcium signaling, synaptic vesicle cycle, glutamatergic synapse
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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