NP137 antibody treatment effects on EMT and metastasis in mouse SCC

Validation Score: 0.900 Price: $0.50 Skin squamous cell carcinoma Primary mouse model Status: proposed

What This Experiment Tests

Validation experiment designed to validate causal mechanisms targeting NTN1 in Primary mouse model. Primary outcome: Proportion of EMT tumor cells, metastasis number, chemotherapy sensitivity

Description

This experiment investigated the therapeutic effects of NP137, a netrin-1-blocking monoclonal antibody, on epithelial-to-mesenchymal transition and metastasis in the mouse skin SCC model. The study administered NP137 to mice with SCC and evaluated multiple outcomes including the proportion of EMT tumor cells, metastasis formation, and chemotherapy sensitivity. Results showed that NP137 treatment decreased EMT tumor cell proportions, reduced metastases, and increased sensitivity to chemotherapy, demonstrating the therapeutic potential of targeting netrin-1 in cancer treatment.

TARGET GENE
MODEL SYSTEM
Primary mouse model
ESTIMATED COST
$0
TIMELINE
0 months
PATHWAY
Netrin-1 signaling pathway
SOURCE
extracted_from_pmid_37532929
PRIMARY OUTCOME
Proportion of EMT tumor cells, metastasis number, chemotherapy sensitivity

Scoring Dimensions

Info Gain 0.00 (25%) Feasibility 0.00 (20%) Hyp Coverage 0.00 (20%) Cost Effect. 0.00 (15%) Novelty 0.00 (10%) Ethical Safety 0.00 (10%) 0.900 composite

📖 Wiki Pages

NTN1 Gene - Netrin 1geneCancerdisease

Protocol

Administration of NP137 monoclonal antibody to mice with skin SCC, followed by assessment of EMT markers, metastasis counting, and chemotherapy response

Expected Outcomes

Decreased EMT, reduced metastases, increased chemotherapy sensitivity

Success Criteria

Significant reduction in EMT tumor cells and metastases with improved treatment response

Related Hypotheses (1)

Netrin-1 Gradient Restoration0.595

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