Validation experiment designed to validate causal mechanisms targeting NEP in APP/PS1 mice. Primary outcome: NEP expression and enzyme activity
In vivo study using APP/PS1 transgenic mice to investigate the effects of AhR agonists on NEP expression and enzyme activity. The experiment tested the same AhR ligands used in the cell culture studies (L-Kynurenine, FICZ, diosmin, and indole-3-carbinol) in a well-established mouse model of Alzheimer's disease. This study aimed to validate the cellular findings in a more physiologically relevant system and determine if AhR activation could have therapeutic potential for Alzheimer's disease through enhanced Aβ clearance mechanisms.
Western blotting, immunofluorescence, quantitative RT-PCR and enzyme activity assay in APP/PS1 mice treated with AhR agonists
AhR agonists would increase NEP expression and enzyme activity in vivo
Significant increase in NEP expression and enzyme activity in brain tissue
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