🧫
Confocal imaging of MSC-chondrocyte interactions during mitochondrial transfer
active
experiment
Created: 2026-04-10T14:35:40
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-aab26845-2b0b-4609-913b-4598a25ef984
🧫 Experiment Protocol
ExploratoryosteoarthritisGJA1human bone marrow-derived MSCs and immortalized human chondrocytesproposed
This experiment used confocal microscopy to visualize the cellular interactions between MSCs and chondrocytes during mitochondrial transfer. Co-cultures were fixed and stained for actin and Cx43 to examine cell-cell contacts and the localization of Cx43 during transfer events. The imaging revealed direct cell contacts between MSCs and chondrocytes, with Cx43 staining enriched at the terminal ends of actin cellular extensions that contained mitochondria in MSCs. This experiment provided morphological evidence for the mechanism of mitochondrial transfer through Cx43-mediated cell-cell contacts and demonstrated the spatial organization of the transfer process.
PRIMARY OUTCOME
visualization of cell-cell contacts and Cx43 localization during mitochondrial transfer
EXPECTED OUTCOMES
Visualization of direct cell contacts with Cx43 enrichment at sites of mitochondrial transfer
SUCCESS CRITERIA
Clear imaging of cell-cell interactions and Cx43 localization
PROTOCOL
Co-culture fixation, immunofluorescent staining for actin and Cx43, confocal microscopy imaging
LINKED HYPOTHESES
Source: PMID 39390589 ↗
🧫 Experiment Extras
PATHWAY
gap junction communication and mitochondrial transfer
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
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0 contradicting
0 neutral
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