Validation experiment designed to validate causal mechanisms targeting HOMER1A,GRIA1,GRIA2,GRIA3,GRIA4 in C57BL/6J mice with stress-induced depression. Primary outcome: Sleep architecture parameters and glutamatergic plasticity markers
This experiment examined sleep regulation and homeostatic glutamatergic plasticity in the medial prefrontal cortex (mPFC) of mice with stress-induced depression-like phenotype. The study characterized altered sleep architecture, impaired sleep homeostasis, and disrupted day-night oscillations of glutamatergic plasticity markers including Homer1a and synaptic AMPAR expression. The mice showed blunted response to sleep deprivation with impaired rebound slow-wave activity, attenuated recovery sleep, and altered upregulation of Homer1a and AMPAR compared to controls.
Sleep monitoring, behavioral assessments, molecular analysis of mPFC tissue for Homer1a and AMPAR expression across day-night cycles
Altered sleep patterns and disrupted circadian oscillations of plasticity markers in depression model
Significant changes in sleep parameters and molecular markers compared to control mice
No related hypotheses
No debates yet
No results recorded yet. Use POST /api/experiments/{id}/results to record a result.