🧫
Generation and characterization of TIGER mice with inducible CD9-GFP
active
experiment
Created: 2026-04-10T23:11:59
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-b02fd7e3-18af-4ea9-9bbd-a292e415f69a
🧫 Experiment Protocol
ValidationCD9Transgenic TIGER miceproposed
A transgenic mouse line was generated containing a CRE-recombinase inducible CAG promoter driving CD9 protein fused to Turbo-GFP (TIGER mouse). The mice were characterized by electroporation with CAG-CRE plasmids or crossing with tamoxifen inducible CAG-CRE-ERT2 or nestin-CRE-ERT2 mice to achieve cell-type specific labeling of extracellular vesicles. CD9-GFP labeled cells were identified as glutamine synthetase and glial fibrillary acidic protein positive astrocytes. This experiment established a novel tool for studying EV biogenesis, release, and cellular uptake in vivo with cell-type specificity.
PRIMARY OUTCOME
Generation of functional CD9-GFP reporter mice for EV tracking
EXPECTED OUTCOMES
Cell-type specific labeling of EVs with CD9-GFP in astrocytes
SUCCESS CRITERIA
Successful CD9-GFP expression in target cells and EV labeling
PROTOCOL
Transgenic mouse generation with CRE-inducible CD9-Turbo-GFP construct, electroporation with CAG-CRE plasmids, crossing with CRE driver lines, immunofluorescence characterization
Source: PMID 30816224 ↗
🧫 Experiment Extras
PATHWAY
Extracellular vesicle biogenesis and tetraspanin signaling
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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