🧫
Small RNA profiling of neonatal astrocyte EVs and microglia response
active
experiment
Created: 2026-04-10T23:11:59
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-cf8ed9a4-cb5d-415d-b2ac-cbdf386d4e6a
🧫 Experiment Protocol
ExploratoryNeonatal astrocyte EVs and microgliaproposed
Neonatal astrocyte-derived extracellular vesicles were analyzed for their small RNA content, revealing a unique signature comprised of morphogenic miRNAs. These EVs were found to induce cytokine release from microglia, suggesting that the small RNA cargo of astrocyte EVs can modulate microglial immune responses. This experiment provided molecular evidence for the immunomodulatory function of astrocyte EVs through their miRNA content, demonstrating a mechanism by which EVs regulate neural immune responses during development.
PRIMARY OUTCOME
miRNA profile of astrocyte EVs and cytokine response in microglia
EXPECTED OUTCOMES
Morphogenic miRNAs in EVs induce microglia cytokine release
SUCCESS CRITERIA
Detection of specific miRNA signatures and cytokine induction
PROTOCOL
EV isolation, small RNA sequencing, microglia treatment with EVs, cytokine analysis
Source: PMID 30816224 ↗
🧫 Experiment Extras
PATHWAY
miRNA-mediated immune modulation and cytokine signaling
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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