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P2rx7 congenic mouse model glucose tolerance testing

active
experiment Created: 2026-04-10T14:45:00 By: etl-v1-backfill Quality: 50% ✓ SciDEX ID: exp-d534102f-47a6-4b79-b55b-224ea58ef3ce
🧫 Experiment Protocol Validationglucose intoleranceP2rx7congenic mice (C57BL6 P2rx7 allele on 129SvJ background)proposed
A congenic mouse model was generated to study purinergic signaling dysfunction by crossing the naturally hypomorphic C57BL6 P2rx7 allele onto the 129SvJ background, creating P2x7-C57 and P2x7-129 comparison groups. Both male and female mice underwent glucose tolerance testing to assess glucose homeostasis. The P2x7-C57 mice (carrying the hypomorphic allele) demonstrated impaired glucose tolerance compared with matched P2x7-129 mice, providing functional evidence for the role of P2X7 in glucose regulation. This model allowed for controlled genetic comparison of P2rx7 function in glucose homeostasis.
PRIMARY OUTCOME
glucose tolerance
EXPECTED OUTCOMES
impaired glucose tolerance in hypomorphic P2rx7 mice
SUCCESS CRITERIA
significant difference in glucose tolerance between P2x7-C57 and P2x7-129 mice
PROTOCOL
generation of congenic mouse strains followed by glucose tolerance testing
🧫 Experiment Extras
PATHWAY
purinergic signaling
MARKET PRICE
$0.50
STATUS
proposed
Metadataorigin_type: v1_polymorphic_backfill
origin_typev1_polymorphic_backfill
source_tableexperiments
_schema_version1
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
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Outgoing
0
0 supporting 0 contradicting 0 neutral
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