Validation experiment designed to validate causal mechanisms targeting P2rx7 in congenic mice (C57BL6 P2rx7 allele on 129SvJ background). Primary outcome: glucose tolerance
A congenic mouse model was generated to study purinergic signaling dysfunction by crossing the naturally hypomorphic C57BL6 P2rx7 allele onto the 129SvJ background, creating P2x7-C57 and P2x7-129 comparison groups. Both male and female mice underwent glucose tolerance testing to assess glucose homeostasis. The P2x7-C57 mice (carrying the hypomorphic allele) demonstrated impaired glucose tolerance compared with matched P2x7-129 mice, providing functional evidence for the role of P2X7 in glucose regulation. This model allowed for controlled genetic comparison of P2rx7 function in glucose homeostasis.
generation of congenic mouse strains followed by glucose tolerance testing
impaired glucose tolerance in hypomorphic P2rx7 mice
significant difference in glucose tolerance between P2x7-C57 and P2x7-129 mice
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