🧫
Conditional CB1R knockout in mPFC CaMKIIα neurons
active
experiment
Created: 2026-04-11T00:49:10
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-dad4506b-fa1d-4cfe-8565-5bcf7b5f64c1
🧫 Experiment Protocol
Validationneuropathic painCNR1,CAMK2ACB1R-floxed mice with conditional knockout in CaMKIIα neuronsproposed
This experiment involved generating conditional knockout mice to specifically delete CB1R from CaMKIIα neurons in the medial prefrontal cortex. Using CB1R-floxed mice crossed with appropriate Cre lines, the researchers created animals lacking CB1R specifically in mPFC excitatory neurons while preserving CB1R expression in other brain regions. The functional consequences of this selective deletion were assessed using behavioral pain testing in the chronic constriction injury model. The study examined how loss of CB1R in this specific neuronal population affects mechanical allodynia and cannabinoid-induced analgesia differently in male and female mice. This approach allowed for precise dissection of the role of CB1R in the mPFC-vlPAG circuit in sex-specific pain modulation.
PRIMARY OUTCOME
Pain sensitivity and cannabinoid analgesia in CB1R conditional knockout mice
EXPECTED OUTCOMES
CB1R deletion in mPFC CaMKIIα neurons causes more severe pain in females; reduced cannabinoid analgesia in knockout mice
SUCCESS CRITERIA
Demonstration of sex-specific effects of CB1R deletion on pain behavior and cannabinoid responses
PROTOCOL
Conditional knockout mouse generation, chronic constriction injury model, behavioral pain testing, cannabinoid administration
LINKED HYPOTHESES
Source: PMID 41380094 ↗
🧫 Experiment Extras
PATHWAY
endocannabinoid signaling, descending pain modulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.