🧫
PFF uptake in primary hippocampal neurons with inhibitors
active
experiment
Created: 2026-04-11T00:48:31
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-dbfdb26b-4a5f-463a-8a23-9e7dbdbe0d1a
🧫 Experiment Protocol
ExploratoryLewy body diseaseSNCAmouse primary hippocampal neuronsproposed
This experiment examined the mechanisms of neuronal uptake of α-synuclein preformed fibrils (PFFs) in primary hippocampal neurons. PFFs were labeled with pH-sensitive dye to track uptake. Neurons were treated with either dynamin inhibitors or clathrin inhibitors to determine which endocytic pathway is responsible for PFF uptake. The study aimed to understand whether PFF uptake occurs through clathrin-mediated endocytosis or dynamin-dependent mechanisms.
PRIMARY OUTCOME
neuronal uptake of PFFs
EXPECTED OUTCOMES
PFF uptake would be blocked by dynamin inhibitors but not clathrin inhibitors
SUCCESS CRITERIA
significant reduction in PFF uptake with dynamin inhibition
PROTOCOL
PFFs labeled with pH-sensitive dye were applied to cultured neurons, with treatment using dynamin or clathrin inhibitors
Source: PMID 41508763 ↗
🧫 Experiment Extras
PATHWAY
endocytosis, protein aggregation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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