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Exosome characterization and miR-934 expression analysis

active
experiment Created: 2026-04-10T22:34:29 By: etl-v1-backfill Quality: 50% ✓ SciDEX ID: exp-dc5b7113-8fbb-4eb1-810b-b0a2b2f618f0
🧫 Experiment Protocol Exploratorycolorectal cancer liver metastasismiR-934CRC cell linesproposed
Transmission electron microscopy and differential ultracentrifugation were used to verify the existence of exosomes derived from colorectal cancer (CRC) cells. The study examined the aberrant overexpression of miR-934 in CRC, particularly in colorectal cancer liver metastasis (CRLM), and analyzed its correlation with poor prognosis in CRC patients. This foundational experiment established the presence and functional relevance of exosomal miR-934 in the context of liver metastasis.
PRIMARY OUTCOME
exosome characterization and miR-934 expression levels
EXPECTED OUTCOMES
confirmation of exosome presence and elevated miR-934 in CRLM
SUCCESS CRITERIA
detection of exosomes and significant miR-934 overexpression
PROTOCOL
transmission electron microscopy, differential ultracentrifugation, expression analysis
🧫 Experiment Extras
PATHWAY
exosome biogenesis
MARKET PRICE
$0.50
STATUS
proposed
Metadataorigin_type: v1_polymorphic_backfill
origin_typev1_polymorphic_backfill
source_tableexperiments
_schema_version1
📊 Evidence Profile
Evidence Balance
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Certainty
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