Tet2 expression analysis in aged 2×Tg-AD mouse brains

Exploratory Score: 0.850 Price: $0.50 Alzheimer's disease APPswe/PSEN1 double-transgenic mice Status: proposed

What This Experiment Tests

Exploratory experiment designed to discover new patterns targeting TET2 in APPswe/PSEN1 double-transgenic mice. Primary outcome: Tet2 expression levels and amyloid plaque burden

Description

This experiment examined the expression levels of Tet2 and amyloid-β plaque accumulation in the brains of aged APPswe/PSEN1 double-transgenic (2×Tg-AD) mice, which serve as a mouse model of Alzheimer's disease. The researchers analyzed brain tissue from these transgenic mice to determine whether there were changes in Tet2 expression associated with amyloid pathology. The study found that aged 2×Tg-AD mice exhibited increased Aβ plaque accumulation alongside decreased Tet2 expression, suggesting an inverse relationship between amyloid burden and Tet2 levels in this AD model.

TARGET GENE
MODEL SYSTEM
APPswe/PSEN1 double-transgenic mice
ESTIMATED COST
$0
TIMELINE
0 months
PATHWAY
amyloid processing/DNA demethylation
SOURCE
extracted_from_pmid_33165916
PRIMARY OUTCOME
Tet2 expression levels and amyloid plaque burden

Scoring Dimensions

Info Gain 0.00 (25%) Feasibility 0.00 (20%) Hyp Coverage 0.00 (20%) Cost Effect. 0.00 (15%) Novelty 0.00 (10%) Ethical Safety 0.00 (10%) 0.850 composite

📖 Wiki Pages

TET2 GenegenePSEN1 Protein (Presenilin-1)proteinPSEN1 Presenilin 1 AD Causal ChainmechanismPSEN1 — Presenilin 1genePSEN1 (Redirect)redirectPSEN1 Mutations in Alzheimer's DiseasediseasePSEN1-Mutant NeuronscellResearchersindexTREM2 Variants in Alzheimer's DiseasediseaseSporadic vs Familial Alzheimer's Disease: ComprehediseasePSEN2 Mutations in Alzheimer's DiseasediseaseProdromal Alzheimer's DiseasediseasePreclinical Alzheimer's DiseasediseaseInvestment Landscape: Alzheimer's DiseasediseaseEarly-Onset Alzheimer's Disease (EOAD)disease

Protocol

Brain tissue analysis from aged 2×Tg-AD mice for Tet2 expression and Aβ plaque quantification

Expected Outcomes

Decreased Tet2 expression correlating with increased amyloid pathology

Success Criteria

Detectable changes in Tet2 levels and amyloid burden

Related Hypotheses (3)

Epigenetic Memory Erasure via TET2 Activation0.515
TET2-Mediated Demethylation Rejuvenation Therapy0.469
Temporal TET2-Mediated Hydroxymethylation Cycling0.408

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