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Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia nigra in PD with unique chromatin signature, and this population normally shows brainstem regional spe
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experiment
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By: experiment_extractor
Quality:
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✓ SciDEX
ID: experiment-exp-2cdb9960aaa3
🧫 Experiment Protocol
Gene Expressionproposed
SUMMARY
Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia nigra in PD with unique chromatin signature, and this population normally shows brainstem regional specificity.
METHODOLOGY NOTES
10x Genomics Chromium platform for single nucleus multiome RNA-seq and ATAC-seq; four brain regions analyzed to compare neurodegeneration severity
N = {'case': 19, 'total': 33, 'units': 'donors', 'control': 14}tissue: substantia nigra, ventral tegmental area, substantia inominata, hypothalamusspecies: homo_sapiens
Related Entities
▸Metadatasource: {'pmid': '37292857', 'type': 'paper', 'e
| source | {'pmid': '37292857', 'type': 'paper', 'extracted_by': 'llm_extraction_v1'} |
| tissue | substantia nigra, ventral tegmental area, substantia inominata, hypothalamus |
| species | homo_sapiens |
| summary | Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia ni |
| sample_size | {'case': 19, 'total': 33, 'units': 'donors', 'control': 14} |
| model_system | postmortem_human_tissue |
| _schema_version | 1 |
| experiment_type | gene_expression |
| methodology_notes | 10x Genomics Chromium platform for single nucleus multiome RNA-seq and ATAC-seq; four brain regions analyzed to compare neurodegeneration severity |
| replication_status | single_study |
| extraction_metadata | {'needs_review': False, 'extraction_date': '2026-04-28T06:19:25.478397+00:00', 'extraction_notes': '', 'extraction_confidence': 0.5} |
| statistical_evidence | None |
📊 Evidence Profile
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