🧫
NPM1-IRF1 protein interaction validation
active
experiment
Created: 2026-04-10T14:35:17
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-131ee8ed-63a2-4580-8582-745a369ba5bc
🧫 Experiment Protocol
ExploratorycancerNPM1, IRF1cultured cellsproposed
Co-immunoprecipitation experiments to verify the physical interaction between NPM1 and the transcription factor IRF1. This experiment aimed to establish the molecular mechanism by which NPM1 regulates antigen presentation by demonstrating direct protein-protein interaction with IRF1, a key transcription factor involved in MHC gene expression regulation.
PRIMARY OUTCOME
protein-protein interaction between NPM1 and IRF1
EXPECTED OUTCOMES
Direct physical interaction between NPM1 and IRF1 proteins
SUCCESS CRITERIA
Successful co-precipitation of NPM1 and IRF1
PROTOCOL
Co-immunoprecipitation assays to detect physical association between NPM1 and IRF1 proteins
LINKED HYPOTHESES
Source: PMID 39402629 ↗
🧫 Experiment Extras
PATHWAY
transcriptional regulation of antigen presentation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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