🧫
HDAC4 inhibition and NHE6 expression mechanism study
active
experiment
Created: 2026-04-10T22:46:49
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-296c7d3d-0631-464d-9fa5-40e2a9450f46
🧫 Experiment Protocol
ExploratoryAlzheimer's diseaseHDAC4, NHE6, LRP1proposed
This experiment investigated the molecular mechanism by which SAHA@LIPO-ANG2 nanoparticles inhibit HDAC4 nuclear translocation and restore NHE6 expression. The study examined how HDAC4 acts as an upstream transcriptional repressor of sodium-hydrogen exchanger 6 (NHE6), and how inhibiting HDAC4 restores NHE6 expression. The researchers validated that restoration of NHE6 expression corrects endosomal hyperacidification and rescues trafficking and plasma membrane expression of LRP1, a key Aβ clearance receptor. This work established the HDAC4-NHE6-pH regulatory axis as a critical pathway in amyloid clearance mechanisms.
PRIMARY OUTCOME
HDAC4 nuclear translocation inhibition and NHE6 expression restoration
EXPECTED OUTCOMES
Inhibition of HDAC4 nuclear translocation would restore NHE6 expression and normalize endosomal pH
SUCCESS CRITERIA
Reduced HDAC4 nuclear translocation, increased NHE6 expression, normalized endosomal pH, restored LRP1 plasma membrane expression
PROTOCOL
Treatment with SAHA@LIPO-ANG2 nanoparticles, assessment of HDAC4 nuclear translocation, measurement of NHE6 expression levels, evaluation of endosomal pH and LRP1 trafficking
LINKED HYPOTHESES
h-4dd0d19b· LRP1-Dependent Tau Uptake DisruptionSDA-2026-04-02-gap-tau-prop-20260402003221-H001· LRP1-Dependent Tau Uptake Disruptionh-seaad-fa5ea82d· APOE Isoform Expression Across Glial Subtypesh-b234254c· TREM2-mediated microglial tau clearance enhancementh-55ef81c5· Extracellular Vesicle Biogenesis Modulation
Source: PMID 41933339 ↗
🧫 Experiment Extras
PATHWAY
HDAC4-NHE6-pH regulatory axis, endosomal pH regulation, LRP1 trafficking
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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