🧫
Brain-derived extracellular vesicles proteome analysis
active
experiment
Created: 2026-04-12T18:03:52
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2eedd52d-50ac-43fd-9d6b-abece6221473
🧫 Experiment Protocol
ExploratoryAlzheimer's disease/tauopathyP2rx7Brain-derived extracellular vesicles from PS19 miceproposed
This experiment analyzed the protein cargo of brain-derived extracellular vesicles (BDEVs) from PS19 mice with and without P2rx7 expression to determine how P2RX7 affects EV cargo loading. Researchers isolated extracellular vesicles from brain tissue and performed proteomic analysis to identify and quantify proteins enriched in EVs, with particular focus on tau and mitochondrial proteins. The study likely involved EV isolation using ultracentrifugation or other purification methods, followed by mass spectrometry-based proteomics. Single-molecule super-resolution microscopy was used to validate findings about tau and mitochondrial protein loading in EVs.
PRIMARY OUTCOME
EV protein cargo composition, particularly tau and mitochondrial proteins
EXPECTED OUTCOMES
P2RX7 increases tau and mitochondrial protein loading in brain-derived EVs
SUCCESS CRITERIA
Significant enrichment of tau and mitochondrial proteins in EVs from P2rx7-expressing mice
PROTOCOL
EV isolation, proteomic analysis by mass spectrometry, single-molecule super-resolution microscopy validation
LINKED HYPOTHESES
Source: PMID 40678243 ↗
🧫 Experiment Extras
PATHWAY
Extracellular vesicle biogenesis and cargo loading
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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