🧫
ChIP-qPCR validation of TFEB binding to Thbs1 promoter
active
experiment
Created: 2026-04-10T22:46:10
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-73188026-b960-47fa-b9fd-21868ba9e676
🧫 Experiment Protocol
Exploratorycardiac fibrosisTFEB, Thbs1mouse cardiac fibroblastsproposed
Chromatin immunoprecipitation followed by quantitative PCR was performed to validate the direct binding interaction between transcription factor TFEB and the promoter region of Thrombospondin-1 (Thbs1). This experiment was conducted to confirm computational predictions that TFEB directly regulates Thbs1 transcription. The assay demonstrated that TFEB physically binds to the Thbs1 promoter region, establishing a direct transcriptional regulatory relationship. This finding was crucial for understanding the mechanism by which TFEB modulates cardiac fibroblast behavior through Thbs1-mediated signaling pathways.
PRIMARY OUTCOME
direct binding of TFEB to Thbs1 promoter
EXPECTED OUTCOMES
TFEB would directly bind to Thbs1 promoter based on computational predictions
SUCCESS CRITERIA
Significant enrichment of TFEB binding at Thbs1 promoter compared to control regions
PROTOCOL
Chromatin immunoprecipitation using TFEB antibody, followed by qPCR amplification of Thbs1 promoter region
LINKED HYPOTHESES
Source: PMID 41935359 ↗
🧫 Experiment Extras
PATHWAY
TFEB-Thbs1 transcriptional regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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