🧫
CB1R expression mapping in mPFC-vlPAG circuit
active
experiment
Created: 2026-04-11T00:49:10
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-a1d18e70-d0a6-403c-8a7b-588547b2a27b
🧫 Experiment Protocol
Exploratoryneuropathic painCNR1,CAMK2A,GAD2GAD2-Cre-tdTomato mice, CB1R-iCre-EGFP miceproposed
This component of the study used advanced molecular techniques to characterize CB1R expression patterns in the medial prefrontal cortex to ventrolateral periaqueductal gray circuit. The researchers employed viral tracing to map projections from mPFC CaMKIIα neurons to vlPAG, combined with immunohistochemistry and fluorescent in situ hybridization to visualize CB1R expression. RNAscope technique was used for high-resolution detection of CB1R mRNA in specific cell populations. The study aimed to identify sex differences in CB1R expression on CaMKIIα-expressing excitatory neurons in the mPFC and determine how these neurons project to and regulate GABAergic neurons in the vlPAG. This mapping was essential for understanding the anatomical substrate underlying sex-specific cannabinoid analgesia.
PRIMARY OUTCOME
CB1R expression patterns and distribution in mPFC-vlPAG circuit
EXPECTED OUTCOMES
Sex-specific differences in CB1R expression on mPFC CaMKIIα neurons and their projections to vlPAG
SUCCESS CRITERIA
Successful visualization and quantification of CB1R expression, identification of projection patterns
PROTOCOL
Viral tracing, immunohistochemistry, fluorescent in situ hybridization, RNAscope technique for mRNA detection
LINKED HYPOTHESES
Source: PMID 41380094 ↗
🧫 Experiment Extras
PATHWAY
endocannabinoid signaling, glutamatergic neurotransmission
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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