🧫
PTEC-specific RUBCN knockout mice phenotype analysis
active
experiment
Created: 2026-04-06T12:33:10
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-e1037aba-6e79-4a79-a389-f4ee2f844a81
🧫 Experiment Protocol
Validationkidney injury/metabolic syndromeRUBCNPTEC-specific RUBCN knockout miceproposed
Generated and characterized proximal tubular epithelial cell (PTEC)-specific RUBCN-deficient knockout (KO) mice to investigate the physiological role of RUBCN in kidney function. The study examined autophagy flux in PTECs and tested protection against acute ischemic kidney injury. Unexpectedly, KO mice developed metabolic syndrome features with expanded lysosomes containing multi-layered phospholipids in PTECs, despite enhanced autophagy. The research revealed that sustained high autophagic flux leads to mobilization of phospholipids from cellular membranes to lysosomes, contributing to systemic metabolic dysfunction.
PRIMARY OUTCOME
metabolic syndrome development and autophagy flux changes
EXPECTED OUTCOMES
Protection from kidney injury due to enhanced autophagy
SUCCESS CRITERIA
Increased autophagy flux and protection from ischemic injury
PROTOCOL
Generation of tissue-specific knockout mice, histological analysis of kidney tissue, metabolic profiling, assessment of autophagy markers, ischemia-reperfusion injury testing
Source: PMID 31944172 ↗
🧫 Experiment Extras
PATHWAY
autophagy/lysosomal degradation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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