🧫
E2F regulation of endocycles in trophoblast giant cells
active
experiment
Created: 2026-04-06T12:29:34
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-faa639b7-f54d-4974-bf5e-82ff546254e2
🧫 Experiment Protocol
ValidationE2F1, E2F2, E2F3, E2F7, E2F8lineage-specific cre mice, trophoblast giant cellsproposed
This experiment used lineage-specific cre mice to investigate the role of E2F transcription factors in regulating endocycles in trophoblast giant cells of the placenta. The study examined how ablation of canonical E2F activators (E2F1, E2F2, E2F3) versus atypical E2F repressors (E2F7, E2F8) affects genome ploidy in these naturally endocycling cells. Trophoblast giant cells undergo endoreduplication cycles, consisting of DNA synthesis and gap phases without cell division, resulting in highly polyploid cells essential for placental development. The researchers used genetic knockout approaches to selectively remove different E2F family members and measured the resulting changes in cellular ploidy levels.
PRIMARY OUTCOME
genome ploidy levels
EXPECTED OUTCOMES
canonical E2F activator ablation increases ploidy, atypical E2F repressor ablation decreases ploidy
SUCCESS CRITERIA
measurable changes in genome ploidy following E2F gene ablation
PROTOCOL
lineage-specific cre-mediated gene ablation in trophoblast giant cells, ploidy measurement
Source: PMID 23064266 ↗
🧫 Experiment Extras
PATHWAY
E2F transcriptional program, cell cycle regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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