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FIGURE 4: NF‐κB signalling upregulates expression of BNIP3L and BNIP3 in ovarian cancer ce...
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Created: 2026-04-22T16:25:26
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ID: paper-fig-d46a46a8-3f6d-499f-aca4-f80e82
FIGURE 4Figure 4
NF‐κB signalling upregulates expression of BNIP3L and BNIP3 in ovarian cancer cells. (A) Effect of RelA/p65 knockdown on BNIP3L and BNIP3 expression in ovarian cancer cells. Spheroid cultured OVCAR3 and OVCAR4 cells were transfected with RelA shRNA; BNIP3L and BNIP3 expression levels were assessed by Western blotting. (B) Effect of NF‐κB inhibitor on BNIP3L and BNIP3 expression in ovarian cancer cells. Spheroid cultured OVCAR3 and OVCAR4 cells were treated with JSH‐23 (10 μM) for 48 h; BNIP3L and BNIP3 expression levels were assessed by Western blotting. (C) The schematic diagram of putative RelA/p65 binding sites in the BNIP3L gene promoter (P1, P2, and P3) that are used for ChIP‐RT‐PCR analysis. P4 in Exon 3 serves as a negative control. (D) ChIP assay demonstrating the binding of RelA/p65 to the BNIP3L gene promoter. Chromatin from spheroid‐cultured OVCAR3 cell lysates was immunoprecipitated using an anti‐p65 antibody. RT‐PCR was conducted to quantify the enrichment of P1–P4 regions
▸Metadata
| doi | 10.1111/jcmm.70704 |
| pmcid | PMC12516242 |
| _origin | {'url': 'https://www.ebi.ac.uk/europepmc/articles/PMC12516242/bin/JCMM-29-e70704-g002.jpg', 'type': 'external', 'tracked_at': '2026-04-22T23:25:26.928368'} |
| caption | NF‐κB signalling upregulates expression of BNIP3L and BNIP3 in ovarian cancer cells. (A) Effect of RelA/p65 knockdown on BNIP3L and BNIP3 expression in ovarian cancer cells. Spheroid cultured OVCAR3 a |
| paper_id | d46a46a8-3f6d-499f-aca4-f80e82536942 |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC12516242/bin/JCMM-29-e70704-g002.jpg |
| image_path | |
| description | |
| figure_label | FIGURE 4 |
| figure_number | 4 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
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