🧫
S-equol effects on TNBC cell proliferation and metastasis in vitro
active
experiment
Created: 2026-04-10T22:47:22
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-08739af4-5a7c-4257-b409-749272837c47
🧫 Experiment Protocol
ExploratoryTriple negative breast cancerPPARγTNBC cell linesproposed
In vitro experiments using triple negative breast cancer (TNBC) cell lines to evaluate the biological effects of S-equol, an active metabolite produced by gut microbiota. The study employed multiple cell-based assays including CCK-8 viability assays, colony formation assays, scratch wound healing assays, and transwell invasion assays to assess the impact of S-equol treatment on cancer cell proliferation and metastatic capacity. The experiments aimed to characterize the direct anticancer effects of S-equol on TNBC cells and establish its potential as a therapeutic compound. Results demonstrated significant suppression of both proliferation and metastatic capacity in TNBC cells following S-equol treatment.
PRIMARY OUTCOME
Cell proliferation and metastatic capacity
EXPECTED OUTCOMES
Suppression of TNBC cell proliferation and metastasis
SUCCESS CRITERIA
Significant reduction in cell viability, colony formation, migration, and invasion
PROTOCOL
CCK-8 assays, colony formation assays, scratch assays, transwell invasion assays
Source: PMID 41925800 ↗
🧫 Experiment Extras
PATHWAY
PI3K/AKT/mTOR signaling pathway, ferroptosis pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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