S-equol effects on TNBC cell proliferation and metastasis in vitro

In vitro experiments using triple negative breast cancer (TNBC) cell lines to evaluate the biological effects of S-equol, an active metabolite produced by gut microbiota. The study employed multiple cell-based assays including CCK-8 viability assays, colony formation assays, scratch wound healing assays, and transwell invasion assays to assess the impact of S-equol treatment on cancer cell proliferation and metastatic capacity. The experiments aimed to characterize the direct anticancer effects of S-equol on TNBC cells and establish its potential as a therapeutic compound. Results demonstrated significant suppression of both proliferation and metastatic capacity in TNBC cells following S-equol treatment.