🧫
Dual-luciferase assay for miR-137-3p target validation
active
experiment
Created: 2026-04-10T22:32:39
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2c1ab059-72a9-4ba0-b592-282f8dbd9ad5
🧫 Experiment Protocol
ExploratoryschizophreniaNr3c1cell culture system with dual-luciferase reportersproposed
An in vitro dual-luciferase reporter assay was conducted to experimentally validate the predicted interaction between miR-137-3p and Nr3c1 mRNA. This functional assay was designed to confirm the in silico prediction that miR-137-3p regulates Nr3c1 gene expression. The dual-luciferase system allows for direct measurement of microRNA-mediated regulation of target mRNA by comparing luciferase activity in the presence and absence of the microRNA. This experiment served as a crucial validation step to confirm the computational predictions regarding miR-137-3p regulation of Nr3c1 expression changes observed in the haloperidol-exposed offspring hippocampus.
PRIMARY OUTCOME
miRNA-target interaction validation
EXPECTED OUTCOMES
Confirmation of miR-137-3p regulation of Nr3c1 expression
SUCCESS CRITERIA
Significant change in luciferase activity indicating miRNA-target interaction
PROTOCOL
Dual-luciferase reporter assay with miR-137-3p and Nr3c1 target sequences
LINKED HYPOTHESES
Source: PMID 35859315 ↗
🧫 Experiment Extras
PATHWAY
microRNA-mediated gene regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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